Homologous recombination in procaryotes
نویسندگان
چکیده
منابع مشابه
Designing E1 Deleted Adenoviral Vector by Homologous Recombination
Adenoviruses are used extensively to deliver genes into mammalian cells, particularly where there is a requirement for high-level expression of transgene products in cultured cells, or for use as recombinant viral vaccines or in gene therapy. In spite of their usefulness, the construction of adenoviral vectors (AdV) is a cumbersome and lengthy process that is not readily amenable to the generat...
متن کاملHomologous recombination
DNA damage, in the form of DNA double-strand breaks, poses a considerable threat to genomic integrity and cell survival. If left unrepaired, a single double-strand break is sufficient to cause cell death and, if repaired inappropriately, a double-strand break may give rise to a potentially oncogenic translocation. Double-strand breaks in genomic DNA may arise accidentally in a number of ways, i...
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Homologous recombination can be divided into three key steps: strand exchange, branch migration and resolution. The identification of a protein complex that catalyses branch migration and Holliday junction resolution argues that the mechanism of homologous recombination is conserved from bacteria to man.
متن کاملHomologous recombination in mammalian cells.
Mouse LTKcells were transfected with a pair of defective Herpes simplex virus thymidine kinase ( t k ) genes. One tk gene had an 8-bp insertion mutation while the second gene had a 100-bp inversion. Extrachromosomal homologous recombination leading to the reconstruction of a functional tk gene was monitored by selecting for tk positive cells using medium supplemented with hypoxanthine/ aminopte...
متن کاملOrientation dependence in homologous recombination.
Homologous recombination was investigated in Escherichia coli with two plasmids, each carrying the homologous region (two defective neo genes, one with an amino-end deletion and the other with a carboxyl-end deletion) in either direct or inverted orientation. Recombination efficiency was measured in recBC sbcBC and recBC sbcA strains in three ways. First, we measured the frequency of cells carr...
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ژورنال
عنوان ژورنال: Microbiological Reviews
سال: 1988
ISSN: 0146-0749
DOI: 10.1128/mr.52.1.1-28.1988